Gene Transfer by DNA/Glycosylated Polylysine Complexes into Human Blood Monocyte-Derived Macrophages
Identifieur interne : 000411 ( France/Analysis ); précédent : 000410; suivant : 000412Gene Transfer by DNA/Glycosylated Polylysine Complexes into Human Blood Monocyte-Derived Macrophages
Auteurs : Patrick Erbacher [France] ; Marie-Thérèse Bousser [France] ; Jacques Raimond ; Michel Monsigny [France] ; Patrick Midoux [France] ; Annie Claude Roche [France]Source :
- Human Gene Therapy [ 1043-0342 ] ; 1996-04-10.
Abstract
Macrophages are putative target cells for expressing an exogenous gene with therapeutical effects. Knowing that macrophages express membrane lectins mediating endocytosis of their ligands, DNA/glycosylated polylysine complexes were used to transfect human blood monocyte-derived macrophages. Monocytes from human peripheral blood were matured in culture for 7 days to differentiate into macrophage-like cells in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). Adherent cells, which displayed characteristic macrophage markers, CD 14, CD 11b, HLA-DR, and HLA-ABC antigens and mannose receptor, were transfected by DNA/glycosylated polylysine complexes in the presence of chloroquine. The luciferase reporter gene expression was maximal 24 hr after transfection with a DNA/mannosylated polylysine complex and by using plasmids in which the promoters (either the long terminal repeat of the human immunodeficiency virus or the human cytomegalovirus) drove the luciferase gene expression. Luciferase gene expression was lower when the promoter was the early region of the large T antigen of SV40 virus. Transfection mediated by DNA/mannosylated polylysine complexes was much more efficient than with DEAE-dextran or lipofectin. The possibility of transferring and expressing an exogenous gene into macrophage-like cells by using a nonimmunogenic synthetic vector as a DNA carrier opens new ways to develop nonviral gene therapy strategies.
Url:
DOI: 10.1089/hum.1996.7.6-721
Affiliations:
- France
- Centre-Val de Loire, Région Centre
- Orléans
- Centre Val de Loire Université, Université d'Orléans
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- to stream France, to step Extraction: 000411
Links to Exploration step
Hal:hal-02161390Le document en format XML
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<author><name sortKey="Midoux, Patrick" sort="Midoux, Patrick" uniqKey="Midoux P" first="Patrick" last="Midoux">Patrick Midoux</name>
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<desc> <address> <addrLine>Rue Charles Sadron 45071 ORLEANS CEDEX 2</addrLine>
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<author><name sortKey="Roche, Annie Claude" sort="Roche, Annie Claude" uniqKey="Roche A" first="Annie Claude" last="Roche">Annie Claude Roche</name>
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<orgName>Université d'Orléans</orgName>
<orgName type="acronym">UO</orgName>
<desc> <address> <addrLine>Château de la Source - Avenue du Parc Floral - BP 6749 - 45067 Orléans cedex 2</addrLine>
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<country>France</country>
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<idno type="DOI">10.1089/hum.1996.7.6-721</idno>
<series><title level="j">Human Gene Therapy</title>
<idno type="ISSN">1043-0342</idno>
<imprint><date type="datePub">1996-04-10</date>
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<front><div type="abstract" xml:lang="en"> <p>Macrophages are putative target cells for expressing an exogenous gene with therapeutical effects. Knowing that macrophages express membrane lectins mediating endocytosis of their ligands, DNA/glycosylated polylysine complexes were used to transfect human blood monocyte-derived macrophages. Monocytes from human peripheral blood were matured in culture for 7 days to differentiate into macrophage-like cells in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). Adherent cells, which displayed characteristic macrophage markers, CD 14, CD 11b, HLA-DR, and HLA-ABC antigens and mannose receptor, were transfected by DNA/glycosylated polylysine complexes in the presence of chloroquine. The luciferase reporter gene expression was maximal 24 hr after transfection with a DNA/mannosylated polylysine complex and by using plasmids in which the promoters (either the long terminal repeat of the human immunodeficiency virus or the human cytomegalovirus) drove the luciferase gene expression. Luciferase gene expression was lower when the promoter was the early region of the large T antigen of SV40 virus. Transfection mediated by DNA/mannosylated polylysine complexes was much more efficient than with DEAE-dextran or lipofectin. The possibility of transferring and expressing an exogenous gene into macrophage-like cells by using a nonimmunogenic synthetic vector as a DNA carrier opens new ways to develop nonviral gene therapy strategies.</p>
</div>
</front>
</TEI>
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</settlement>
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<tree><noCountry><name sortKey="Raimond, Jacques" sort="Raimond, Jacques" uniqKey="Raimond J" first="Jacques" last="Raimond">Jacques Raimond</name>
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<country name="France"><region name="Région Centre"><name sortKey="Erbacher, Patrick" sort="Erbacher, Patrick" uniqKey="Erbacher P" first="Patrick" last="Erbacher">Patrick Erbacher</name>
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<name sortKey="Bousser, Marie Therese" sort="Bousser, Marie Therese" uniqKey="Bousser M" first="Marie-Thérèse" last="Bousser">Marie-Thérèse Bousser</name>
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<name sortKey="Monsigny, Michel" sort="Monsigny, Michel" uniqKey="Monsigny M" first="Michel" last="Monsigny">Michel Monsigny</name>
<name sortKey="Roche, Annie Claude" sort="Roche, Annie Claude" uniqKey="Roche A" first="Annie Claude" last="Roche">Annie Claude Roche</name>
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